Inhibitory Activities and Secondary Structures of Two Types of Soybean Trypsin Inhibitors

Abstract

Abstract: The inhibition curves of I-KSTI and BBTI against trypsin showed that the concentrations to attain 50% inhibitory ratio of trypsin activity were about 1.5μg/ml and 1.2μg/ml for I-KSTI and BBTI respectively. Complete inhibition concentrations against trypsin for BBTI and I-KSTI were 13.5μg/ml, 9μg/ml。In the presence of I-KSTI and BBTI, the Km value of trypsin was kept unchanged at 5.88×10-4 mol/L for benzoyl-DL Arginine-p-nitroanilide substrate while the Vmax was decreased. The far-UV CD (circular dichroism) spectra of both I-SKTI and BBTI showed a single negative peak at around 200nm and the negative minimum was measured as [θ]200nm=-2545deg.cm2/dmol for I-SKTI and [θ]200nm=-797deg.cm2/dmol for BBTI. The secondary structure of I-KSTI was composed of β-sheet (22.5%), β-turn (16.2%) and random (61.4%) whereas BBTI was composed only of β-sheet (52.6%) and random (47.4%).

Key words: soybean trypsin inhibitors, circular dichroism, secondary structure

HUANG Hui-Hua, LIANG Han-Hua, GUO Qian-Chu. Inhibitory Activities and Secondary Structures of Two Types of Soybean Trypsin Inhibitors[J]. FOOD SCIENCE, 2005, 26(3): 46-49.

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Inhibitory Activities and Secondary Structures of Two Types of Soybean Trypsin Inhibitors

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