Study on Extraction, Purification Identification and Physical and Chemical Characteristics Assay of Rhodiola Henryi Polysaccharides

Abstract

Abstract: The R. Henryi Polysaccharides (RHP) were isolated from R. henryi by a high efficient water-extrating method. RHP- I,RHP-II and RHP-III were obtained by ethanol precipitating in RHP. After using RHP-III, eliminating albumen, decolorating, dialysis and freeze drying, it was purified by DEAE-cellulose column chromatography. As purified polysaccharides, RHPs were obtained and identified by Saphadex G-200 column chromatography and UV and IR spectrum.The results analysed by paper chromatography showed that its monnosaccharides were arahinose(Ara), rhamnose (Rha) and glucose(Glc), but a monnosaccharide should be further identified.

Key words: R.Henryi polysaccharides(RHP), extraction, purification, identification, physical and chemical charac- teristics

LIU Zhi-Wei, JIANG Hua-Nian. Study on Extraction, Purification Identification and Physical and Chemical Characteristics Assay of Rhodiola Henryi Polysaccharides[J]. FOOD SCIENCE, 2005, 26(3): 60-63.

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Study on Extraction, Purification Identification and Physical and Chemical Characteristics Assay of Rhodiola Henryi Polysaccharides

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