FOOD SCIENCE ›› 2005, Vol. 26 ›› Issue (5): 119-122.

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Isolation of Acidα-Amylase Producing Strain and Purification and Characterization of Acidα-Amylase

 CHEN  Bo, LI  Da-Li, YANG  Shu-Lin   

  1. Department of Biology Engineering, Nanjing University of Science and Technology
  • Online:2005-05-15 Published:2011-09-19

Abstract: A strain of acidα-amylase producing fungi was isolated from soil. It was identified as Penicillium. The optimum temperature of acidα-amylase 60℃ and the optimum pH 4.4. The molecular weight of the purified acid α-amylase was about 85kD. The results of thin-layer chromatography demonstrated that the products from starch catalyzed by the enzyme were oligosaccharide and dextrin. This enzyme should be classified as anα-amylase.

Key words: Penicillium, acidα-amylase, purification