Studies on the Production and Purification of Staphylococcal Enterotoxin B

Abstract

Abstract: By the method of cellophane covered agar plate culture, production of staphylococcal enterotoxin B was studied. After incubation at 37℃ for 48h, cells were harvested and the cell suspension was centrifuged at 10000×g for 15min at 4℃. The supernatant was condensed by polyglycol 20000. Then condensation was purified to electrophoretic homogeneity of the staphylococcal enterotoxin B by ion-exchange chromatography on CM-cellulose and by gel filtration on Sephadex G-200. This method was simple and could be applicable for laboratory.

Key words: Staphylococcus aureus, staphylococcal enterotoxin B, purification, ion-exchang chromatography, gel filtration

WANG Xiao-Hong, XIE Bi-Jun, SHI Xian-Ming, SUN Ke, LI Wei. Studies on the Production and Purification of Staphylococcal Enterotoxin B[J]. FOOD SCIENCE, 2005, 26(5): 88-91.

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Studies on the Production and Purification of Staphylococcal Enterotoxin B

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