Abstract: Arthrobacter nicotinovorus (JNU-1)can produce trehalose synthase enzyme. Study finds that trehalose synthase enzyme is a kind of enzyme in the cell. After substantive cultivation of bacterium JNU-1 and enrichment of culture mycelium, the cells harvested were disrupted by ultrasonic, followed by leach and centrifugation to obtain crude enzyme solution. The crude enzyme was fractionated with ammonium sulfate and the active fractions were collected, dialyzed, concentrated and successively purified by column chromatography packing Sephadex G-100. The purity and molecular weight of the enzyme were determined by SDS polyacrylamide gel electrophoresis.

Key words: Arthrobacter nicotinovorus, trehalose synthase enzyme, purification, property