Abstract: Two separation methods of cholesterol were compared in food contained egg: one was conventional Soxhlet method; the other was supercritical carbon dioxide extraction(338 bar,40℃) along with solid phase separation( a solid phase collector was joined with ODS static phase). Egg noodles, biscuits and sweetmeat were equably mixed, and then was added in the internal standard and anhydrous Na2SO4. One part of evey sample was separated by SFE-SPE method, and the remainder was done against benzinum purificatum by conventional method. After Soxhlet separation, the further purification was made by SPE. Dissocia- tive cholesterol can be determined in the Soxhlet-SPE and SFE-SPE separation (with capillary gas chromatography). The same result was gained by two separation methods. However, the continuous SFE-SPE was a substitute for Soxhlet method, for with many important assets such as short analysis time and a few solvent needed.

Key words: supercritical fluid, extraction, separation, purification, egg, cholesterol