Latex Particle Reagent Prepared for of Escherichia coli O157 Shiga-Toxin 1

Abstract

Abstract: The sequence encoding of the mature protein of STX1 A of E.coli O157 strain 94H, isolated from some patient in China, was amplified by PCR. The primers were designed from STX1 toxin gene sequence of E. coli strain 933W. The amplified gene was cloned into prokaryotic expression vector pET-28a. Then the recombinant pET28a-stx1A was transformed into host strain E. coli BL21 (DE3). Expressed recombinant STX1 A was used to immunize rabbits to prepare anti-serum. The IgG fraction of the antiserum was cross-linked with the latex particle suspension to yield a final immunoglobulin. The described methods enabled a sensitive and simple assay of STX1 A production.

Key words: E. coli O157, STX1 A subunit, prokaryotic expression, latex particle reagent

LIU Jian-qing1,ZHOU Zhi-jiang1,HAN Ye1,ZHENG Feng1,BO Qing-ru2. Latex Particle Reagent Prepared for of Escherichia coli O157 Shiga-Toxin 1[J]. FOOD SCIENCE, 2006, 27(1): 29-33.

[1]	XIA Xiaoyu, LI Han, WANG Zhenyu, TAN Xiaoyi, CHENG Shuzhen, DU Ming. Purification and Preparation of Nanoparticles of Human H-chain Ferritin [J]. FOOD SCIENCE, 2020, 41(12): 91-98.
[2]	WANG Jingjing, WANG Ting, ZHANG Xinxiao, BIAN Huan, GENG Zhiming, LI Pengpeng, WANG Daoying, XU Weimin. Expression, Purification and Characterization of 12-Lipoxygenase Catalytic Domain from Pig Muscle [J]. FOOD SCIENCE, 2019, 40(8): 41-47.
[3]	TIAN Wanfan, LIU Ji, ZHAO Yanying, TANG Junni. Prokaryotic Expression, Purification, and Solution Conformation of Staphylococcus aureus Enterotoxin K [J]. FOOD SCIENCE, 2019, 40(4): 138-145.
[4]	LI Liu, ZHENG Zhe, WU Fengyu, HAO Yijiang, ZHAO Xiao, CAO Yongqiang, YU Zhijian, CHEN Chao, YANG Zhennai,. Recombinant Expression and Structural Properties of Milk-Clotting Enzyme from Bacillus methanolicus [J]. FOOD SCIENCE, 2019, 40(22): 39-46.
[5]	ZHAO Jianhua, LI Haoxia, YIN Yue, WANG Yajun, LI Yanlong, FAN Yunfang, AN Wei, CAO Youlong. Cloning and Prokaryotic Expression of the Xylose Isomerase Gene (LbxylA) from Chinese Wolfberry (Lycium barbarum Linn.) Fruit and Preparation of Polycloal Antibody against the Fused Protein [J]. FOOD SCIENCE, 2019, 40(10): 77-83.
[6]	DAI Jieyu, JIANG Yonghua, ZHANG Yujie, WANG Haojie, LIU Xiaoran, LIU Cuihua, REN Xiaolin. Prokaryotic Expression of MdHB1, a Transcription Factor from Malus domestica and Preparation of Polyclonal Antibody against It [J]. FOOD SCIENCE, 2018, 39(8): 69-76.
[7]	ZHANG Yumei, LI Pengpeng, ZHANG Muhan, WANG Jingjing, WANG Daoying, XU Weimin. Prokaryotic Expression, Purification and Identification of Phospholipid Binding Activity of Anas platyrhynchas HSP90α [J]. FOOD SCIENCE, 2018, 39(2): 99-104.
[8]	SUN Ying, PAN Si’an, LI Mengmeng, DENG Weiwei, ZHANG Zhengzhu. Cloning, Prokaryotic Expression and Functional Identification of Guanine Deaminase Genes from Saccharomyces cerevisiae and Escherichia coli [J]. FOOD SCIENCE, 2018, 39(18): 139-144.
[9]	HAN Yanwen, LIAN Shuangqiu, HAN Yunyun, CHI Ming, YAN Shijie. Prokaryotic Expression of POD Gene from ‘Yali’ Pears and Effects of Maturity and Cooling Rate on the Expression of POD Gene during Storage [J]. FOOD SCIENCE, 2017, 38(9): 40-45.
[10]	LIU Ji, YANG Fan, TIAN Wanfan, LONG Hu, SUN Siyu, ZHOU Yushan, ZHAO Yanying, TANG Junni. Prokaryotic Expression, Purification, Identification and Solution Conformation of Staphylococcal Enterotoxin M [J]. FOOD SCIENCE, 2017, 38(24): 40-46.
[11]	YANG Jumei, MA Jianzhong, WANG Yonggang, DENG Zibing, WEI Yan, PAN Bo, LI Yinwu. Epitope Prediction, Cloning, Expression and Immunogenicity of the Coat Protein of Strawberry Mild Yellow Edge Virus [J]. FOOD SCIENCE, 2017, 38(16): 71-78.
[12]	CHEN Xinquan, DU Lihui, JU Xingrong, WU Xueyou, YUAN Jian, HE Rong. Expression and Purification of Pediocin PA-1 in Escherichia coli [J]. FOOD SCIENCE, 2016, 37(3): 97-102.
[13]	DING Chengchao, XIE Manman, ZENG Haijuan, LIU Qing. Gene Sequence Analysis and Polyclonal Antibody Preparation of Outer Membrane Protein, Ompk, in Vibrio [J]. FOOD SCIENCE, 2016, 37(23): 141-146.
[14]	HOU Jinhui, ZHANG Xiang, QIAO Gaoxiang. Cloning, Expression and Activity of an Endo-1,4-β-D-Glucanase from Pantoea ananatis [J]. FOOD SCIENCE, 2016, 37(23): 211-215.
[15]	YANG Yang, LI Rongzhuo, MAO Lugang, LIU Xia*. Detection of E. coli O157:H7 Based on Au Nanoparticles of Different Sizes Labeled Second Antibody Enhanced Surface Plasmon Resonance [J]. FOOD SCIENCE, 2015, 36(8): 201-205.

Latex Particle Reagent Prepared for of Escherichia coli O157 Shiga-Toxin 1

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments