Abstract: Establish a quantitative detection method in micro level of blood on the base of reagent box for detecting the TC, TG, and HDL-C in ordinary amount of blood . Methods: For detection of TC, TG, and HDL-C using two parts of 50μl blood. Each mouse only 110μl of orbit blood can be collected. Using half stuff of the reagent box for serum enzyme reaction and color development, than the reaction liquid is poured into a 600μl cell for TC, TG and HDL-C colorimetric analysis. Results and conclusion: Mice feed on high lipids food for 30 days a hyperlipemia model can be established. By comparison of the preciseness, accuracy and experimental condition with the primitive reagent box detection, it is revealed that the micro amount blood analysis method is reliable.

Key words: inspective method, serum total cholesterol, tri-fatty acid glycerol, high density lipoprotein-cholesterol