Abstract: Egg white was used as raw material,contrast analysis and orthogonal test to do a system research on the optimal disassembling conditions of enzyme in preparing high F value oligopeptides. The DH was regarded as a basis evaluating index. According to tests results of factors such as pH value,hydrolyzing temperature,time and E/S,meanwhile,an orthogonal design of L9(34) was adopted to draw a conclusion suitable for the hydrolysis of Flavouerzyme as followpH7.5,temperature 60℃,E/S 3.3%,5 hours. The optimal conditions for preparing high F value as followactive carbon to liquid 1:10,temperature 50℃,pH 7 and 3 hours.

Key words: exoproteinase, high F value