FOOD SCIENCE ›› 2006, Vol. 27 ›› Issue (11): 229-232.

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Effect of L-malate on Gene Expression of Proteins and Enzymes Related to the Malate-aspartate Shuttle

   Wu-Jun-Lin,   Wu-Qing-Ping,   Wei-Ming-Ken,   Wu-Hui-Qing,   Zhou-Xiao-Yan   

  1. 1.South China Sea Institute of Oceanology,Chinese Academy of Sciences,Guangzhou 510301,China; 2.Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application,Guangdong Institute of Microbiology,Guangzhou 510070,China; 3.Graduate University of the Chinese Academy of Sciences,Beijing 100049,China
  • Online:2006-11-15 Published:2011-11-28

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Abstract:  To investigate the expression change of two key enzymes in mitochondria and carried proteins (AGC and OMC) in inner member of mitochondria related to malate-aspartate shuttle,the expression of these proteins was examined in liver of mice treated L-malate by methods of RT-PCR. RT-PCR was used to analyze the expression of key enzymes (mAST and mMDH) mRNA and carried protein (AGC and OMC) mRNA after treatment of L-malate. The results indicated that the expression of AGC mRNA was increased significantly in the L-malate treated group compared with control group. There were no difference in the levels of mAST,mMDH and OMC mRNA. The results predict a potential molecule mechanism that L- malate increased capability of the malate-aspartate shuttle by enhancing the level of AGC mRNA.

Key words: L-malate, malate-aspartate shuttle, carried protein