Abstract: The preparation,purification and characterization of free radical scavenging active peptides from collagen hydrolysates of Paracaudina chinens var. were researched. Bromelain was used to hydrolyze the sea cucumber collagen. The optimal hydrolytic conditions of sea cucumber collagen with Bromelain were determined by orthogonal test. The results show that the optimal hydrolytic conditions of Bromelain are temperature 40℃,enzyme dosage240U/ml,concentration of substrate 18mg/100ml(as hydroxyproline),pH5.2,time 4h. And the hydrolysate prepared has about 52.20% of the scavenging ratio on O·2. The high free radical scavenging active fractions were separated and purified using the ultrafiltration and Sephadex G-25.

Key words: Paracaudina chinens var., collagen, enzyme hydrolysis, peptide, free radical