Separation of the Isomers of Red Carotenoid in Nature Red Yolk with Reverse-phase C18-HPLC

FOOD SCIENCE ›› 2006, Vol. 27 ›› Issue (11): 473-478.

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Separation of the Isomers of Red Carotenoid in Nature Red Yolk with Reverse-phase C18-HPLC

LIU Liang-Zhong, Zhang-Sheng-Hua, Shi-Jia-Yi, Wei-Ze-Yi

1.College of Food Science and Technology,Wuhan Polytechnology University,Wuhan 430023,China; 2.College of Food Science and Technology,Huazhong Agricultural University,Wuhan 430070,China; 3.College of Life Science,Yangtze University,Jingzhou 434103,China; 4.Wuhan Disease Prevent and Control Center,Wuhan 430020,China

Online:2006-11-15 Published:2011-11-28

Abstract

Abstract: The red carotenoid was separated into 5 peaks by HPLC with 5μm,25cm×4.6mm ODS RP-C18 column,flow phase: methanol-water(99.5:0.5 V/V),flow velocity: 0.5ml/min,sample size: 5μl,determinating wavelength 480nm,column temperature: 18℃. The 5 peaks had the same molecular weight 562 identified by ESI-MS. The results indicated that the red carotenoid could be separated into 5 isomers by RP-C18-HPLC with the conditions above.

Key words: RP-C18-HPLC, mass spectrum separation, carotenoid, nature red yolk

LIU Liang-Zhong, Zhang-Sheng-Hua, Shi-Jia-Yi, Wei-Ze-Yi. Separation of the Isomers of Red Carotenoid in Nature Red Yolk with Reverse-phase C18-HPLC[J]. FOOD SCIENCE, 2006, 27(11): 473-478.

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Separation of the Isomers of Red Carotenoid in Nature Red Yolk with Reverse-phase C18-HPLC

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