Abstract: A metabolic pathway model of γ-linolenic acid was constructed in this paper. On the basis of the model, metabolic flux distributions of γ-linolenic acid biosynthesis at different fermentation stages and the reasons of the changes were analyzed. The analysis showed that the key nodes of the metabolic control were G6P, PYR and AcCoA. The metabolic flux of γ-linolenic acid could be increased by adding NaAC which could adjust the AcCoA node.

Key words: metabolic flux； &gamma, -linolenic acid； biosynthesis；