FOOD SCIENCE ›› 2006, Vol. 27 ›› Issue (12): 33-37.

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Application and Activity Assay of Co-expressed Organophosphorus Hydrolase and Carboxylesterase(B1) Produced by Genetic Engineering Bacteria

ZHENG Yu-zhi1,GUO Cheng-jun2,ZHANG Jian-liang1, GUO Xin-min2,QIAO Chuan-ling1   

  1. 1.Institute of Zoology, State Key Laboratory of Integrated Management of Pest Insects and Rodents, Chinese Academy of Science, Graduate University of Chinese Academy of Sciences, Beijing 100080, China;2. School of Environment, Renmin University of China, Beijing 100872, China
  • Online:2006-12-15 Published:2011-11-23

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Abstract: Carboxylesterase and organophosphorus hydrolase (OPH) are capable of hydrolyzing and detoxifying different pesticides. In order to get the widest substrate spectrum organophosphorus hydrolase and carboxylesterase (B1) were co-expressed in one genetic engineering bacteria. The activity of OPH was assayed by orthogonal test, and the optimum condition were substrate concentration 2×10-4mol/L, temperature 25℃, reaction time 10min and pH8.0. In the detoxification of parathion on mosquitoes, 95% of the mosquitoes can be survived. And it has been used in biosensor for pesticide detection.

Key words: carboxylesterase, organophosphorus hydrolase, enzyme activity assay