Abstract: With the single-factor experiment and the orthogonal design, various extracting conditions for total paeony glycoside (TPG) were observed. The results showed that the optimum extracting conditions of ethanol under reflux was 70% ethanol with 10 times of solvent to extract twice, once for 30min, and the extraction rate of TPG was 4.68%; the best technique of supersonic wave extraction was 70% ethanol with 14 times of solvent to extract twice, once for 30min, the extraction rate of TPG was 4.47%. Also, the purification of TPG by macro adsorption resin was investigated. The results showed that nonpolar resin ATD-21 and D101 were good sorbents of TPG. The optimum conditions of ATD-21 included that the concentration of sample was 2. 1～4.2mg/ml with flow rate of 1.0～3.0ml/min, the quantity of sample was 52.5mg/g resin and 30% ethanol 60ml was used as eluant. With the optimum technological process, the collection ratio of TPG was 82.9% and the purity reached 74.1%.

Key words: total paeony glycoside (TPG), extraction, purification, macro adsorption resin