Purification and Characterization of Phycoerythrin from Bangia fusco-purpurea

Abstract

Abstract: Phycoerythrin was isolated and purified from Bangia fusco-purpurea. After the successive procedures of ammonium sulfate fraction precipitation, ion-exchange column chromatography, and gel filtration chromatography, the purity (A565/A280) was 5.0. Absorption spectrum characteristic of the purified PE showed that it is R-PE. Relative molecular weight of subunits consisting of R-PE was detected by SDS-PAGE followed by silver staining. The stability of R-PE was investigated under a broad range of pH, different temperatures and light exposure.

Key words: Bangia fusco-purpurea, phycoerythrin, purification, stability

FU Xiao-ping, ZHOU Qiong, WENG Ling, SU Wen-Jin, CAO Min-Jie. Purification and Characterization of Phycoerythrin from Bangia fusco-purpurea[J]. FOOD SCIENCE, 2006, 27(12): 482-486.

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Purification and Characterization of Phycoerythrin from Bangia fusco-purpurea

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