Isolation,Purification and Analysis of Glycoprotein from Soybean

Abstract

Abstract: A soybean glycoprotein was isolated and purificated from soybean by using DEAE-Cellulose 52 IEC(Ion-exchange Chromatography) and Sephadex G-200 SEC(Size-exchange Chromatography). The soybean glycoprotein was analysed by SDS-PAGE for its purity and molecular mass.The content of the soybean glycoprotein is 30.55% and the molecular mass is 61000.β-elimination reaction indicated the linkage type of the soybean glycoprotein is O-glycosidic linkage and IR Spectra is α-glycosidel inkage.

Key words: soybean glycoprotein, isolation, purification, structure analysis

ZHAN Ling, PAN Si-Yi. Isolation,Purification and Analysis of Glycoprotein from Soybean[J]. FOOD SCIENCE, 2006, 27(12): 594-596.

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Isolation,Purification and Analysis of Glycoprotein from Soybean

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