Abstract: A GPC-HPLC method for the determination of Para Red and Sudan Red Dyes in capsicum and capsicum products was described. Para Red and Sudan Red Dyes was extracted from samples with cyclohexane-ethyl acetate (1:1, V/V). The extract was cleaned up with gel permeation Chromatography. The analysis was performed on a Diamond column (250mm×4.6mm i.d.5μm) with acetonitrile-water (95:5, V/V) as the mobile phase at a flow rate of 1.0ml/min, detection at 500 nm by UV. The calibration curves present good linear relationship in the range of 0.01～10μg/ml, and the correlation coefficients were above 0.9998 for all compounds. Recoveries of all dyes added were 90%～102%respectively. Detection limit of the method for Para Red and Sudan Red Dyes was 0.01mg/kg.

Key words: gel permeation chromatography(GPC), high performance liquid chromatography(HPLC), capsium, Para Red, Sudan Red Dyes