Abstract: By a series of functionality experiments, the functionality of the fermented supernatant of Laozao (rice distillers’ grain) was studied in this paper, such as to reduce the risks of the Alzheimer disease (AD), antioxidation, hypotension, hypoglycemic. The inhibition effects of α-glucosidase and acetylcholinesterase(AChE) were validated. Then, the inhibition effects of the fermented supernatant of different producing areas and different fermented time and different diluted concentra- tions were studied onα-glucosidase, while the inhibition effect of ethanol was excluded. The fermented supernatant was boiled and centrifuged and the activity of α-glucosidase was assayed by p-NPG(p-nitrophenyl α-D-glucopyranoside). It showed that in primal concentration, the fermented supernatant of the different producing areas had steady inhibition effect on α-glucosidase daring the course of fermentation of Laozao, it also had steady inhibition effect on α-glucosidase and the inhibition rate was higher than 60 percent. Moreover,in the sixtieth to sixty-sixth hour, the inhibition effect is highest. In addition, the different concentrations of fermented supernatant of Laozao had different inhibition effect. When the concentration increased, the inhibition effect increased significantly. This finding suggests that the fermented supernatant of Laozao has steady inhibition effect on α-glucosidase.

Key words: the fermented supernatant of Laozao(rice distillers&rsquo, grain)； &alpha, -glucosidase； inhibition； hypoglycemic；