Studies on Extraction Technology of Total Steroidal Saponins from Fenugreek Seeds by Cellulase

Abstract

Abstract: Using enzyme-extracting methods, the optimum extraction conditions of steroidal saponins from the of fenugreek seed flour were investigated by orthogonal design with the extracting rate of steroidal sapogenin as index. The results showed that the factors affecting extraction efficiency of steroidal saponins were as follows: the enzyme dosage>treatment temperature > ratio of solid to liquid (g/ml)>treatment time. The percentage of extraction of steroidal sapogenin could be obviously raised by enzymic treatment, the average value was 83.6% for enzyme-extracting method and 58.4% for the same condition without cellulase. The extracting percentage of steroidal saponins from fenugreek seeds could be improved by cellulase treatment.

Key words: Trigonella foenum-graecum L, steroidal saponins, enzymic treatment, extraction process

ZHANG Li-Ming, LI Xia, XU Wei. Studies on Extraction Technology of Total Steroidal Saponins from Fenugreek Seeds by Cellulase[J]. FOOD SCIENCE, 2006, 27(2): 157-160.

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Studies on Extraction Technology of Total Steroidal Saponins from Fenugreek Seeds by Cellulase

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