FOOD SCIENCE ›› 2006, Vol. 27 ›› Issue (2): 160-162.

Previous Articles     Next Articles

Study on Cell Breaking Extraction of Ribonucleic Acid from Brewer Yeast

ZHU  Jun-Dong, HUANG  Guo-Rong, MI  Man-Tian, LANG  Hai-Bin   

  1. Department of Nutrition and Food Hygiene,Third Military Medical University
  • Online:2006-02-15 Published:2011-09-05

Abstract:  In this study,two different methods of extracting ribonucleic acid from brewer yeast by using salt and high pressure homogenization for breaking brewer yeast cell wall.The results showed that the extraction rate of RNA was 2.21% by using salt for breaking brewer’s yeast cell wall by the conditions: salt concentration 8%, temeperature 121℃, time 30 minutes and pH2. The yield rate of RNA is 3.08% by using high pressure homogenization for breaking brewer’s yeast cell wall according to the conditions: working pressure 70MPa and 3 times homogenization.

Key words: brewer yeast, salt, high pressure homogenization, ribonucleic acid