Assaying Total Flavonoids in Black Corn Pollen by Double-wavelength Spectrophotometry

FOOD SCIENCE ›› 2006, Vol. 27 ›› Issue (2): 230-232.

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Assaying Total Flavonoids in Black Corn Pollen by Double-wavelength Spectrophotometry

ZHANG Lan-Jie, XIN Guang, ZHANG Wei-Hua

Department of Chemistry, Anshan Normal University, Anshan 114005, China

Online:2006-02-15 Published:2011-09-05

Abstract

Abstract: In this paper, a double-wavelength spectrophotometry was applied to assay the total flavonoids in black corn pollen. The detective wavelength was 510 nm ,while the reference one was 584 nm, and the recovery rate was 98.14%, with RSD=1.24% (n=5).The results indicated that the average total flavonoids content in black corn pollen was 3.53%, and the method was stable, reliable and accurate in the assay process.

Key words: black corn pollen, total flavonoids, double-wavelength spectrophotometry

ZHANG Lan-Jie, XIN Guang, ZHANG Wei-Hua. Assaying Total Flavonoids in Black Corn Pollen by Double-wavelength Spectrophotometry[J]. FOOD SCIENCE, 2006, 27(2): 230-232.

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Assaying Total Flavonoids in Black Corn Pollen by Double-wavelength Spectrophotometry

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