Abstract: A simple convenient and rapid method of capillary electrophoresis for the separation and analysis of the chiral hesperidin enantiomers was established. The effects of different chiral selectors and their concentration, buffer pH and its concentration, methanol concentration, and voltage and temperature on the separation of chiral hesperidin enantiomers were studied. The optimized conditions were: 12mmol/L of hydroxypropyl-β-cyclodextrin, 200mmol/L of borate-methanol solution in ratio of 7:3 and pH9.7 at separation voltage 23kV and temperature 30℃. The baseline separation of hesperidin enantiomers is achieved with a resolution factor of 1.50.

Key words: capillary electrophoresis； hesperidin； hydroxypropyl-&beta, -cyclodextrin； chiral separation；