FOOD SCIENCE ›› 2006, Vol. 27 ›› Issue (5): 36-40.

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Research on Conditions of Ultrasonic Wave Disruption of Two Lactobacillus sp. Cells for Extraction of Linoleate Isomerase

CAO  Jian, ZHANG  Hao, YU  Hai-Dong, WANG  Yue-Nan, WANG  Hong-Jun,   Wang-Yu-Jun, XIANG  Li-Xin   

  1. College of Biological Engineering, Henan University of Technology, Zhengzhou 450052, China
  • Online:2006-05-15 Published:2011-09-16

Abstract:  Ultrasonic wave was used in this paper to disrupt the cells of two Lactobacillus species so as to extract linoleate isomerase from the cells, and the effects of the disruption conditions to release linoleate isomerase were studied. After single-factor experiment and orthogonal experiment, the optimum disruption conditions for Lactobacillus acidophilus were: intermittent ultrasonic treatment 80 times (time for each treatment was 3s, with a 4s interval), volume of the treated cell suspension 50ml, concentration of cell suspension 20g/60ml, pH value of the extraction buffer 4.0, and concentration of NaCl 2.0mol/L; while the optimm conditions for Lactobacillus bulgaricus were: intermittent ultrasonic treatment 80 times (time for each treatment was 3s, with a 4s interval), volume of the treated cell suspension 60ml, concentration of cell suspension 15g/50ml, pH value of the extraction buffer 4.8, and concentration of NaCl 3.0mol/L.

Key words: Lactobacillus acidophilus, Lactobacillus bulgaricus, linoleate isomerase, ultrasonic wave disruption, condition