Abstract: The crosslinking-carboxymethyl double modified glucan(CCMG)was prepared by the yeast glucan with the reaction of chloroacetic acid and epichlorohydrin in presence of alkaline. The optimal reaction conditions were determined by a single- factorial experiment and orthogonal experiment with the index of degree of substitution (DS). The results indicated that the optimum conditions are as follows: reaction temperature 50℃, reaction time 5h , chloroacetic acid 3.0g and NaOH 50%.The DS is about 0.5 under these conditions. Compared with β-D-glucan, CCMG showed a very good efficacy on the adsorption of ZEA. Its affinity to ZEA is up to 18.643μg/mg CCMG, which is greater than that of β-D-glucan with only 2.296μg/mg.

Key words: crosslinking-carboxymethylglucan(CCMG), double-modified, zearalenone(ZEA), adsorption