FOOD SCIENCE ›› 2006, Vol. 27 ›› Issue (8): 227-231.

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An Indirect Competitive ELISA Method for Quantitative Analysis of Staphylococcal Enterotoxin B

 WANG  Xiao-Hong,   Xu-Kang,   Wang-Ying,   Wang-Ru-Kai,   Shi-Xian-Ming,   Xie-Bi-Jun   

  1. 1.College of Food Science and Technology,Huazhong Agricultural University,Wuhan 430070,China;2.College of Agriculture and Biology,Shanghai Jiaotong University,Shanghai 201101,China
  • Online:2006-08-15 Published:2011-10-14

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Abstract: Staphylococcal enterotoxin B was adopted to immune Balb/c mouse.When the mouse serum titer was appropriate,the Balb/c mouse was injected with a non-antibody-secreting myeloma cell SP2/0 for the preparation of antibody.An indirect competitive ELISA for detecting SEB was established in this study.The detection limit of indirect ELISA assay established in this study is 1ng/ml with a detection concentration range of 1～103ng/ml and correlation coefficient R2=0.9951.The recovery of artificially contaminated sample is 94.5%.

Key words: staphylococcal enterotoxin B, anti-SEB antibody, indirect competitive ELISA