FOOD SCIENCE ›› 2006, Vol. 27 ›› Issue (8): 56-59.

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Analysis of Phenolic Substances in Transgenic Soybean Seeds and Other Soybean Varieties

CAO  Bai-Ying, 欧Shi-Yi , HUANG  Cai-Huan, 欧Yun-Fu , SUN  Xi, MA  Ji-Feng   

  1. 1.Department of Food Science and Engineering,Jinan University,Guangzhou 510632,China;2.Testing Center of Jinan University,Guangzhou 510632,China;3.College of Agronomy,Hunan Agricultural University,Changsha 410128,China
  • Online:2006-08-15 Published:2011-10-14

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Abstract: The safety of genetically modified soybean(mainly Roundup Ready soybean)aroused worldwide concern and much work on its safety evaluation was carried out.Glyphosate-tolerant soybean(RR soybean)is a soybean variety that introduces an exogenous gene which controls expression of 5-enolpyruvylshikimate-3-phosphate synthase(EPSPS).EPSPS is a key enzyme in the secondary metabolism that would affect phenolic substances metabolism.It suggests that metabolism of phenolic substances would change with respect to its parent.In this research,RR soybean and its parent,two none-genetically modified soybean varieties were used to test the phenolic substances by HPLC in the seeds of different fraction,including oil,protein and pulp.The results showed that there were significant differences in composition and content of phenolic substances between the four kinds of materials,including between RR soybean and its parent.The results suggested that the fingerprint of phenolic substances may play a role in safety evaluation of soybean variety and genetically-modified soybean.

Key words: RR soybean, phenolic substances, HPLC spectrogram