Abstract:  Kombucha is a kind of symbiosis with yeast and acetic acid bacterium. In this paper, effects of ultimate pH value and cellulosic pellicle membrane were studied with difference tea-brothes, carbon sources, nitrigen sources, initial pH values, ion intensities, temperatures, cultivating modes in tea fungus fermentation with traditional cellulosic pellicle as fermentation starter. The results were showed as following: supplement of ethanol and peptone, pan cultivation have prominence effects on forming cellulosic pellicle, the final pH value has negative correlation with forming cellulosic pellicle.

Key words: kombucha, cellulosic pellicle