FOOD SCIENCE ›› 2007, Vol. 28 ›› Issue (1): 161-164.

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Breeding of CGTase Processing Strain with N+ Ion Implantation

 WANG  Yan-Ping, CHEN  Lin-Hai, LI  Zong-Wei, QIN  Guang-Yong, LI  Zong-Yi, TAN  Zhong-Fang, DUAN  Yu-Hang, HUO  Yu-Ping   

  1. 1.Henan Provincial Key Labortory of Ion Beam Bioengineering, Zhengzhou 450052, China; 2.College of Life Science, Henan Normal University, Xinxiang 453007, China
  • Online:2007-01-15 Published:2011-12-31

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Abstract:  With N+ ion implantation, a high-yield cyclodextrin glucanotransferase processing strain Bacillus sp.HA-1 was selected, with CGTase activity level 1.6 times of that of original strain. Its CGTase activity reached 6800IU/ml when cultivated for 24 hours in 5m3 fermentor. Using the CGTase to transform 25% tapioca starch intoβ-CD, the convertion rate in 12 hours is 85%. The CGTase activitise of F2～F20 are ranged from 98.11% to 102.66% as compared with F1, which proves that the genetic quality of B. sp. HA-1 strain is stable.

Key words: N+ ion beam implantation； cyclodextrin glucanotransferase； &beta, -cyclodextrin； breeding； fermentation；