FOOD SCIENCE ›› 2007, Vol. 28 ›› Issue (1): 73-76.

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Study on Germination and Inactivation of B.subtilis Spores in Fresh Milk by Oscillatory and Pulsed Ultra High Pressure

 DE  Li-Ge-尔Sang, WANG  Ying-Li, A  La-Da-Ri-Tu, MA  Li-Na   

  1. 1.College of Food Science and Engineering, Inner Mongolia Agricultural University, Huhhot 010018, China; 2.College of Bioengineering, Inner Mongolia Agricultural University, Huhhot 010018, China
  • Online:2007-01-15 Published:2011-12-31

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Abstract: A method of germinating and sterilizing B.Subtilis spores with minimal heating was studied by the combined effect of oscillatory and pulsed UHP, pressurization times, and with certain operations cycles. The condition above room temperature was benefit to UHP sterilization. At 40℃, seven pressurization cycles with the first pressure at 200MPa for 5min, followed by an ambient pressure pause for 1min, then another 700MPa pressurization for 5min, decreases the spores count from 107 to 102～ 103cells/ml.

Key words: ultra high oressure(UHP), B.Subtilis spores, spore germination, spore inactivation