Abstract: Salidroside nano-liposomes were prepared by using 4 different methods: thin film evaporation, ultrasound method, reverse phase evaporation, or freezing-thawing method. The effects of different preparation methods and salidroside concentra- tions on the formation of liposomes were examined by means of encapsulating efficiency. The experimental results showed that the encapsulating efficiency of liposomes prepared by different methods varies in the order: freezing-thawing method > reverse phase evaporation > ultrasound method > thin film evaporation. The optimization conditions of the reverse phase evaporation were as follows: the loading efficiency of salidroside reaches 40%, the ratio of cholesterol and lecithin is 1:2 (W/W) and Tween 80 added 2%, and the encapsulating efficiency 80% with pH7.0, 0.05 mol/L PBS.

Key words: Rhodiola sachalinensis A. Bor, Salidroside nano-liposomes, preparation encapsulating efficiency, reverse phase evaporation