Abstract: Objective: The influences factors of drawing yolk antibody has been researched, the best environment of drawing the yolk antibody and the method of drawing the yolk antibodies the most have been look for. Methods: the yolk antibody has been drew under 1:1 dilution fold, different PEG6000 density, different temperature, under PBS of different pH values, the protein content which is also called antibody content has been measured with the ultraviolet spectrophotometer; the abstraction amount has been compared separately under different environment. Results: The PEG6000 density, environment temperature, and PBS of different pH values on extraction of yolk IgY vary remarkablely. The best density of drawing PEG6000 of the yolk antibody is about 4%, the best pH values is 7.0 and the best drawing temperature about 31 degrees centigrade,the difference between pH value 7.0 and pH value 6.8 is not notable.

Key words: egg yolk antibody, abstraction, PEG6000 density, pH value, environment temperature