FOOD SCIENCE ›› 2007, Vol. 28 ›› Issue (11): 443-445.

Previous Articles     Next Articles

Determination of Total Alkaloid in Zanthoxylum nitidum(Roxb.)DC. by Bromophenol Blue Colorimetry

 LONG  Sheng-Jing, CHEN  Yao, PENG  Xing   

  1. Department of Chemistry, Guangxi Medical University, Nanning 530021, China
  • Online:2007-11-15 Published:2011-11-22

Abstract: The content of total alkaloid in Zanthoxylum nitidum(Roxb.) was determined by bromophenol blue colorimetry, and the UV-visible spectrophometry was adopted to determine the total alkaloid with the wavelength at 393 nm. The total alkaloid is in linear relationship within the concentration range of 2.56~ 10.26μg/ml(r=0.9997), the average recovery rate is 101.00% (RSD=2.46%, n=6). Bromophenol blue colorimetry is simple, quick and accurate in the determination of total alkaloid in ZanthoxyLum nitidum (Roxb.)DC.

Key words: ZanthoxyLum nitidum(Roxb.)DC, total alkabid, bromophenol blue colorimetry