Abstract: Hesperidin is a bioflavonoid with many biological activities. The adsorption conditions, exchange reaction behavior, reaction kinetics and thermodynamics were investigated by batch ion-exchange method. The results showed that the optimum adsorption condition is at 60 ℃, pH 11.5 and hesperidin concentration 2.43 mg/ml, and the resin with little diameter is adaptableto adsorb hesperidin because of its large specific surface area. The adsorption process in solution exhibits Freundish behaviour. In addition, the exchange rate constant at 60 ℃ is 0.5167 h-1, which decreases as the temperature decreases, and the exchange activation energy Ea is 23.45 kJ/mol. Furthermore, the apparent equilibrium constant Ke of D296 for hesperidin is 7336.15 g2/L2. So the exchange reactions can occur spontaneously forΔG0 -24.63kJ/mol, greater than zero. Then the reaction enthalpy ΔHm0 is 69.69 kJ/mol, indicating the reaction endothermic. And the entropy increases during the reaction for ΔS0 0.28 kJ/mol, greater than zero.

Key words: hesperidin, ion-exchange resin, adsorption, kinetics, thermodynamics