Comparison of Separation and Purification Methods between Intra-and Extra-cellular Acidic Polysaccharides from Microcystic aeruginosa and Identification of Their Products

Abstract

Abstract: Microcystic aeruginosa, a kind of blue-green alga, is the main alga in algae bloom. In this study extra-and intra-cellular crude polysaccharides were purified from M.aeruginosa by different temperature and pH. Their acidic components which had alduronic acid groups were purified by DE-52, and then acidic polysaccharides of the two groups from intra-cellular and one group from extra-cellular were purified by Sephadex columns. Each of these three acidic polysaccharides presents a single HPLC peak in its respective chromatographs of DE-52 and Sephadex G-150 columns. These three acidic polysaccharides are different from each other in their molecular weights, alduronic acid contents and monosaccharides constitutents.

Key words: Microcystic aeruginosa, intracellular acidic polysaccharide (IAPS), extracellular acidic polysaccharide (EAPS), isolation, identify

ZHANG Ling, ZHOU Bin-Wei, MEI Qiu-Hong, WANG Xi-Da, LU Chang-Mei, WU Guo-Rong. Comparison of Separation and Purification Methods between Intra-and Extra-cellular Acidic Polysaccharides from Microcystic aeruginosa and Identification of Their Products[J]. FOOD SCIENCE, 2007, 28(12): 244-248.

[1]	WANG Wei, LI Jinjin, CHI Hai. Purification and Antimicrobial Mechanism of Amylocyclicin W5 Produced by Bacillus amyloliquefaciens DH8030 [J]. FOOD SCIENCE, 2021, 42(7): 29-34.
[2]	GONG Min, MA Yanqiu, WANG Ruihong, CHI Yuan, CHI Yujie. Isolation and Identification of Typical Bacterial Strains from Eggshell Surface and Evaluation of Their Spoilage Potential in Liquid Whole Egg [J]. FOOD SCIENCE, 2021, 42(4): 153-160.
[3]	ZHANG Yu, WANG Fang, WENG Zebin, BAO Yifan, SONG Haizhao, SHEN Xinchun. Screening of Wheat Germ Albumin-Derived Antioxidant Peptides and Its Protective Activity against Cellular Oxidative Damage [J]. FOOD SCIENCE, 2021, 42(17): 10-18.
[4]	ZHANG Nana, CHEN Lirong, ZHANG Shoumei, GUO Yuqiu, LIU Kaichang, GONG Kuijie. Isolation and Identification of Fungal Contaminants and Analysis of Mycotoxin Content in Fresh Corn during Grain Development [J]. FOOD SCIENCE, 2021, 42(16): 273-280.
[5]	LIU Pei, CHEN Cheng, QIN Xinguang, ZHANG Haizhi, HU Zhongze, WU Qiong, YAO Renyong, WANG Xuedong, LIU Gang. Emulsifying Properties of Different Kinds of Aggregates from Whey Protein Isolate and Stability of Pickering Emulsions Stabilized with Them [J]. FOOD SCIENCE, 2021, 42(12): 24-30.
[6]	LIU Jingbo, WANG Ziqin, YU Yiding, ZHANG Ting, LIU Boqun. Identification and Mechanism Analysis of Angiotensin-I Converting Enzyme Inhibitory Peptides from Soybean Meal [J]. FOOD SCIENCE, 2021, 42(12): 123-129.
[7]	CHEN Hong, ZHANG Qian, CHEN Yulei, WENG Ling, ZHANG Lingjing, XIE Shaohao, LIU Guangming, CAO Minjie. Preparation and Activity Analysis of DPP-IV Inhibitory Peptides from Pacific Oyster (Crassostrea gigas) [J]. FOOD SCIENCE, 2021, 42(10): 120-126.
[8]	XUE Hongkun, TAN Jiaqi, LIU Chai, LIU Chenghai. Isolation, Purification, Structure Identification and Anti-tumor Activity of Anthocyanin from Kyoho Grape Skins [J]. FOOD SCIENCE, 2020, 41(5): 39-48.
[9]	MENG Fanbing, WANG Zhongwei, LI Yuncheng, MA Changzhong, LIU Dayu. Isolation of Salt-tolerant and Aroma-producing Yeasts from Soybean Paste Starter and Their Fermentation Characteristics [J]. FOOD SCIENCE, 2020, 41(24): 31-38.
[10]	ZHAO Rui, SHAO Changchun, GAO Shigong, LIU Guangrui, YANG Fumin. High-throughput Sequencing Analysis of Bacterial Diversity of Different Types of Traditional Chinese Bacon [J]. FOOD SCIENCE, 2020, 41(20): 90-96.
[11]	ZHENG Yi, ZHANG Jian, CAO Yongqiang, ZHAO Xiao, YU Zhijian, CHEN Chao, YANG Zhennai. Isolation, Identification and Enzymatic Characteristics of Kluyveromyces marxianus Producing β-D-Galactosidase [J]. FOOD SCIENCE, 2020, 41(2): 158-165.
[12]	SHAO Shuangyu, SI Xiali, ZHANG Yansong, TU Pengfei, ZHANG Qingying. Recent Advances in Analytical Methods for Polysaccharides from Edible Mushroom [J]. FOOD SCIENCE, 2020, 41(19): 272-280.
[13]	LIU Bingbing, ZHOU Kaixin, YE Lijing, YU Yingying, LI Xuesi, YAN Peixun, GUO Shuxian. Identification and Flavor Analysis of an Aroma-producing Bacterium Isolated from Fermented Grains of Strong Flavor Baijiu in Songhe Town [J]. FOOD SCIENCE, 2020, 41(18): 105-112.
[14]	QU Yun, TONG Yao, TAN Yongping, ZHAO Yanying, TANG Junni. Epidemiological Characteristics of Staphylococcus aureus Isolates Collected during Yak Slaughter [J]. FOOD SCIENCE, 2020, 41(17): 169-175.
[15]	ZHAO Lumiao, GAO Haiyan, LIU Ruiling, DING Yuting, HAN Yanchao, CHEN Hangjun. Identification of Dominant Postharvest Pathogens of Taro and Antifungal Activity of Hinokitiol against Them [J]. FOOD SCIENCE, 2020, 41(12): 136-142.

Comparison of Separation and Purification Methods between Intra-and Extra-cellular Acidic Polysaccharides from Microcystic aeruginosa and Identification of Their Products

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments