Purification and Specific Properties of Recombinant L-sorbose/L-sorbosone Dehydrogenase

Abstract

Abstract: L-sorbose/L-sorbosone dehydrogenase produced by gene engineering strain Y517# can transform L-sorbose to 2-KGA, the Vitamin C precursor. By means of ammonium sulfate precipitation, DEAE Sepharose Fast Flow and Q Sepharose High Performance assay, a purified recombinant L-sorbose/L-sorbosone dehydrogenase was obtained from Y517#. The results showed that the molecular weight of the purified enzyme is of 66 kD. The optimum temperature and pH are 40 ℃ and 7.0 respectivily. The enzyme activity is stable at pH 6.0～9.0 and below 45℃.

Key words: recombinant L-sorbose/L-sorbosone dehydrogenase, gene engineering strain, purification, enzyme properties

XIE Li, ZHANG Duo, GUO Hui-Can, ZHANG Li-Ping, LI Man, ZHAO Bao-Hua. Purification and Specific Properties of Recombinant L-sorbose/L-sorbosone Dehydrogenase[J]. FOOD SCIENCE, 2007, 28(12): 278-282.

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Purification and Specific Properties of Recombinant L-sorbose/L-sorbosone Dehydrogenase

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