Abstract: Objective: To study the antioxidant activities of acid proteoglycan from Folium mori (APFM). Methods: In chemical mimicking experiments, the scavenging of hydroxyl free radical (·OH) and superoxide anion free radical (O2·) by APFM was determined. The effects of APFM on the production and accumulation of malondialdehyde (MDA) in mice organs induced by ·OH was examined, while also, the inhibition of APFM on the swelling of mice liver mitochondria induced by· HO was assayed. Through establishing mice model experiments, the effects of APFM on MDA content and superoxide dismutase (SOD) in the organs of diabetic mice induced by alloxan were observed and analyzed. Results: APFM can efficiently scavenge ·OH and O2· produced in chemical mimicking system, inhibit the production and accumulation of MDA both in mice organs induced by ·HO and in the organs of diabetic mice induced by alloxan, prominently lower the swelling of mice liver mitochondria induced by ·OH and increase the SOD activity in dose-dependent manner. Conclusion: APFM has strong antioxidant activity in vitro and in vivo, so it is an efficient free radical scavenger.

Key words: Folium mori, acid proteoglycan, antioxidant activity