FOOD SCIENCE ›› 2007, Vol. 28 ›› Issue (2): 30-33.

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Separation, Purification and Physicochemical Analysis of the Hydroxylpropyl Oligo-konjac Glucomannan Sulfate

 HUANG  Hao, GAN  Xin   

  1. College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430068, China
  • Online:2007-02-15 Published:2011-12-31

Abstract: The oligo-konjac glucomannan sulfate product was prepared after alcohol sediment twice, with dialysis and vacuum freezing drying. By determining respectively the contents of sulfate and sugar, the sulfated sample Ⅰand non-sulfated sample Ⅱwere obtained by gel filtration. The sample Ⅰappeared as a single band by separax electrophoresis. The weight average of relative molecular weight of hydroxypropyl sulfated oligo-konjac glucomannan is 1595D, the number average of relative molecular weight is 1572D, and PDI (MW/MN) is 1.01 by GPC.

Key words: oligo-konjac glucomannan, sulfating, purification