Abstract: The variations of free amino acids of excised tea leaves under anoxic stress were studied. The results showed that: The total content of free amino acids of excised tea fresh leaves is 2.5%. The Gabaron endemic amino acids of tea-theanine are determinated besides the normal protein amino acids. The free amino acids in high contents are Glu, Pro, Asp, Arg, His,while in low contents are Thr, Ser, Gly. The total content of free amino acids of excised tea leaves is raised to 3.4% after 3h anoxic state. The contents of Pro, Gaba, Gly, Ser, Ala, Val, Thr are increased with the contents of Pro and Gaba even 3 and 15 times higher than those in the normal condition respectively, but the contents of Glu with Asp are reduced, with the rates respectively 45% and 36%. The contents of the other amino acids are stable.

Key words: anoxic stress, excised tea fresh leaves, free amino acids