FOOD SCIENCE ›› 2007, Vol. 28 ›› Issue (4): 211-217.

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Glutathione Isolation and Purification of Yeast Extract with Ion Exchange Resin Columns

 WANG  Miao, FAN  Chong-Dong, SU  Xiao-Jin   

  1. Key Laboratory of Food Science and Safety,Ministry of Education,School of Food Science and Technology,Southern Yangtze University,Wuxi 214036,China
  • Online:2007-04-15 Published:2011-12-31

Abstract: Glutathione,one of the most important biological active substance plays a central role in metabolic pathways of live organisms.In this paper,A,B two cation ion-exchange columns were used to isolate and purify of GSH from yeast extract.The technology parameters of both A and B were:for column A,sample pH 5.5~6.0,for column B,sample pH 2.6~2.8,column B eluate 1.2% NaCl.The recovery rate of GSH is more than 70%,while the GSH has been purified more than 100 times.

Key words: glutathione(GSH), ion-exchange, isolation and purification