Building Thermotolerant High-alcohol-producing Yeast by Proplast Fusion

Abstract

Abstract: As chemical drugs and UV used to exterminate the single parent protoplasts were choosen as hereditary markers, with 0.01% triadimefon content and irradiated by UV 2 rain,the optimum conditions of the orthogonal test are:33.3%(W/V) concentration of crude corn in fermenting material,400 U/g of glucoamylase(per gram of raw corn),9%(V/V)inoculum of yeast, and initial pH 5.5.The orthogonal test indicated that the fusion strain LZ-3 produces the highest amount of alcohol,or 9.0% alcohol after incubation at 42℃for 72 hours.

Key words: protoplast, fusion, thermotolerant, high-alcohol-producing yeast

LU Ling-Ling, WANG Min, ZHU Hui-Xia, SUN Jin-Xu, TAN Jian-Xin, ZHANG Wei. Building Thermotolerant High-alcohol-producing Yeast by Proplast Fusion[J]. FOOD SCIENCE, 2007, 28(4): 231-236.

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Building Thermotolerant High-alcohol-producing Yeast by Proplast Fusion

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