Optimizing Conditions for Enzymolysis of Crude Peptide from Rape Pollen by Response Surface Methodology

FOOD SCIENCE ›› 2007, Vol. 28 ›› Issue (7): 117-121.

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Optimizing Conditions for Enzymolysis of Crude Peptide from Rape Pollen by Response Surface Methodology

HU Xiao-Bo, XU Ming-Gang, LIU Zhi-Wei, WU Mou-Cheng, LIU Li

1.College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; 2.School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 201101, China; 3.National Institute of Nutrition and Food Safety; Chinese Center for Disease Control and Prevention, Beijing 100050, China

Online:2007-07-15 Published:2011-10-24

Abstract

Abstract: Based on single-factor tests analysis (substrate concentration, enzyme concentration and substrate concen- tration ratio, pH, enzymolysis time and enzymolysis temperature), the optimum conditions of alcalase enzymatic hy- drolysis are determined by response surface methodology as follows: pH9, hydrolyzing temperature 50 ℃, enzyme concentration 1460 U/g pro substrate concertration 6%, hydrolyzing time 2 h.

Key words: enzymatic hydrolysis, rape pollen gluntelin, response surface methodology

HU Xiao-Bo, XU Ming-Gang, LIU Zhi-Wei, WU Mou-Cheng, LIU Li. Optimizing Conditions for Enzymolysis of Crude Peptide from Rape Pollen by Response Surface Methodology[J]. FOOD SCIENCE, 2007, 28(7): 117-121.

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Optimizing Conditions for Enzymolysis of Crude Peptide from Rape Pollen by Response Surface Methodology

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