Abstract: For the purpose of providing information and screening microorganism for agriculture production and microecologics production, 22 strains Bacillus isolated from soil, feedstuff and organic fertilizer were identifited according to the handbook of Berges bacteriology identification and their physiologic and biochemical test. The result demonstrate that, T-2, T-6, T-8, S-6, S- 4, T-1 were Bacillus choshinensis, Bacillus paenibacillus chondroitinus, Bacillus paenibacillus amyloyticus, Bacillus paenibacillus kobensis, Bacillus paenibacillus pabuli, Bacillus paenibacillus polymyxa, respectively. The rest 16 strains Bacillus are on the study. On the basis, antibiotic susceptibility of the six Bacillus strains were detected to 35 antibiotics of 12 groups by the agar diffusion test. The results demonstrated that, all strains examined were sentive to quinoline group, aminoglycoside group, macrolide group and peptide group, and resistant to sulphonamide group, while showing different antibiotic susceptibility to the other antibiotics.

Key words: Bacillus, isolation, identification, antibiotic susceptibility testing