Abstract: Objective: To screen AFM1 mimic epitope by using phage display peptide library technology. Methods and Results: After three rounds of biopanning, 4 positive phage clones designated as A1, A2, A3 and A4 were pick out and found that all of them could block the binding of AFM1 to antigen when the pfu of phage was 2.5 ×1010. The inhibition rates were 41.8%, 40.6%, 36.7% and 37.6% respectively. Results of DNA analysis showed that A1 and A2 belong to the same clone and their identical amino acid sequence is LTSFPRH; A3 and A4 belong to the same clone and their iolentical amino acid sequence is MAPSSWR. Conclusions: 2 AFM1 mimic epitopes were obtained from phage display peptide library.

Key words: AFM1, mimic epitope, phage display library