Abstract: Objective: A high performance liquid chromatographic method was developed to determine sucralose. Method: The column of DIKMA C18 (250 mm × 4.6 mm i.d. 5 μm) and a mobile phase of methanol-water (65:35, V/V) at a flow rate of 1.0 ml/min and column temperature 40 ℃ with refractive index detector were adopeted. Results: The method is proved to be linear in the range of sucralose from 1.5～2000 mg/L. The recovery is 99.65%. The relative standard deviation (RSD) is 0.15%. Conclusion: The method is simple, fast and stable. It is suitable for quantitative analysis for sucralose production process.

Key words: high performance liquid chromatography, sucralose, refractive index detector