FOOD SCIENCE ›› 2007, Vol. 28 ›› Issue (9): 103-106.

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Study on Hemagglutination Properties of Lectins from Tremella fuciformis

 SHI  Guo-Han, MA  Ai-Min, WU  Yao, TAN  Jia-Yu   

  1. College of Food Science and Technology,Huazhong Agricultural University,Wuhan 430070,China
  • Online:2007-09-15 Published:2011-11-22

Abstract: The development of Tremella fuciformis contained three stages of growth,namely spore,mycelium and fruiting body.The isolation procedure involved extraction dealt with phosphate buffered saline and precipitation of 30%~60%(NH4)2SO4.These three lectins from the three stages are all less thermostable.When heated at 70 ℃,they lose their original activity.The agglutination activity of the spore lectin is only stable in neutral environment,whereas the lectins of mycelium and fruiting body are alkali-and acid-stable respectively.Their activities are affected by both demetalization and addition of metal ions including Mg2+,Al3+,Fe3+.Among the various carbohydrates tested,lactose is the only saccharide capable of inhibiting the agglutinating activity of mycelium lectin.

Key words: Tremella fuciformis Berk, lectin, hemagglutination