Abstract: Objective:To establish a high performance liquid chromatography method for determination of 6 kinds of synthetic colour in jelly.Methods:6 kinds of synthetic colour were separated on a C18 column using acetonitrile:ammonium acetate as the mobile phase at a flow rate of 1.0 ml/min,with the column temperature 25℃ and detection wave 254 nm and 300 nm.Results:The detection limits were 0.07～0.16 mg/kg,the calibration curves were liner in the range of 1.0～50.0 mg/L,the precision was not more than 8.5% and the rate of recovery were 98.5% to 100.6%.Conclusion:The method established in the present paper is simple,precise,fast and suitable for determination of 6 kinds of synthetic colour in jelly.

Key words: jelly, chromatography, liquid, synthetic colour