Study on Optimization of Extraction and Purification of Anthocyanidins from Vaccinium uliginosum L. by RP-HPLC

Abstract

Abstract: Depending on the detection of RP-HPLC,the change trend of the extraction rate of anthocyanins from Vaccinium uliginosum L. with the hydrochloric acid concentration was investigated. The best hydrochloric acid concentration is determined as 1.5 mol/L. The Vaccinium uliginosum L.extracts were purified by AB-8 macroporous resin,polyamide resin (eluted with 80% or 40% ethanol solution) and combination of the two resins,respectively,and four anthocyanins samples were obtained and then detected by RP-HPLC. The results showed that the anthocyanins purity of the four samples are 13.36397%,36.95687%,13.21466% and 8.559135%,respectively. According to the above results,the polyamide resin is apparently better than AB-8 macroporous resin in the purification of anthocyanins,and the anthocyanins purity is increased by 23.5%.

Key words: RP-HPLC, Vaccinium uliginosum L., anthocyanins

DONG Yi, LIN Song-Yi, LIU Yan-Feng, LI Ming-Jin, ZHANG Xing-Mao, LIU Xuan, LIU Jing-Bo. Study on Optimization of Extraction and Purification of Anthocyanidins from Vaccinium uliginosum L. by RP-HPLC[J]. FOOD SCIENCE, 2008, 29(10): 349-352.

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Study on Optimization of Extraction and Purification of Anthocyanidins from Vaccinium uliginosum L. by RP-HPLC

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