Comparison of Enzymatic Properties between Purified Lactase and Lactase in Permeabilized Cells

Abstract

Abstract: Two lactases were prepared by permeabilied cell treatment and extraction combined with purification methods separately. The enzymatic properties of the two lactases were analyzed and compared. The results showed that their optimum pHs and temperatures are similar respectively. The pH stability and thermal stability of the lactase in permeabilized cells are greater than those of the purified enzyme respectively. Both the activities of the two lactases are enhanced by metal ions,such as K+,Mn2+,Mg2+and Ca2+,whereas they are strongly inhibited by Cu2+,and the purified enzyme activity is more greatly affected by metal ions than lactase in permeabilized cells. Maltose,glucose and other sugars have inhibitory effects on both of the two enzymes. The Km values and Vmax values of the two of lactases determined under the same reaction conditions are different.

Key words: lactase, permeabilized cells, purification, enzyme properties

YAN Ya-Li, WANG Chang-Lu, LIU Hui-Juan, CHEN Qing-Sen, LIU Jian-Fu. Comparison of Enzymatic Properties between Purified Lactase and Lactase in Permeabilized Cells[J]. FOOD SCIENCE, 2008, 29(10): 436-439.

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Comparison of Enzymatic Properties between Purified Lactase and Lactase in Permeabilized Cells

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