Abstract: The crude sapogenins extracted from dried "Enwuyemi" Gynostemma pentaphyllum leaves were recrystallized and separated by Sephadex G-15 to obtain sapogenin monomers. According to the detection results by mass spectrometer,the obtained 3,7,12-hydroxy-24-acetyl-sapogenin is of the molecular weight 435 D and the most possible molecular formula C26H43O5,whose 17th place is combined with octane having ester group. And 7-8-carbonyl-11-hydroxy-3-acetyl-sapogenin is also found,whose molecular weight and most possible molecular formula are 501 D and C32H53O4 respectively,and whose 3rd place is combined with ester group. Both of them are identical with the parent nucleus of original variant of Gynostemma pentaphyllums apogenins,and are tipical ginseng sapogenins. The combination of 17th or 15th place ofthe two formers with octane and the combination of 17th place of the latter with octane might reveal the reason of their no bitterness.

Key words: "Enwuyemi", Gynostenma pentaphyllum； sapogenins； isolation and identification；